Deep optoacoustic localization microangiography of ischemic stroke in mice

Super-resolution optoacoustic imaging of microvascular structures deep in mammalian tissues has so far been impeded by strong absorption from densely-packed red blood cells. Here we devised 5 μm biocompatible dichloromethane-based microdroplets exhibiting several orders of magnitude higher optical absorption than red blood cells at near-infrared wavelengths, thus enabling single-particle detection in vivo. We demonstrate non-invasive threedimensionalmicroangiography of the mouse brain beyond the acoustic diffraction limit (<20 μm resolution). Blood flow velocity quantification in microvascular networks and light fluence mapping was also accomplished. In mice affected by acute ischemic stroke, the multi-parametric multi-scale observations enabled by super-resolution and spectroscopic optoacoustic imaging revealed significant differences in microvascular density, flow and oxygen saturation in ipsi- and contra-lateral brain hemispheres. Given the sensitivity of optoacoustics to functional, metabolic and molecular events in living tissues, the new approach paves the way for non-invasive microscopic observations with unrivaled resolution, contrast and speed.


Xosé Luís Deán-Ben
Justine Robin
Daniil Nozdriukhin
Ruiqing Ni
Jim Zhao
Dr. Chaim Glück
Jeanne Droux
Juan Sendón-Lago
Zhenyue Chen
Quanyu Zhou
Prof. Dr. Bruno Weber
Susanne Wegener
Anxo Vidal
Michael Arand
Dr. Mohamad El Amki
Daniel Razansky

Next publication

The bumpy road of purinergic inhibitors to clinical application in immune-mediated diseases